01152nas a2200229 4500008004100000245010100041210006900142300001000211490000900221520048900230653001500719653000800734653001200742653001500754653001300769653001500782653001600797653001400813100001700827700003500844856004300879 2008 eng d00aA new species of Hedyselmis Hinton and notes on the phylogeny of the genus (Coleoptera: Elmidae)0 anew species of Hedyselmis Hinton and notes on the phylogeny of t a55-620 v17813 a

A new species, Hedyselmis belatani sp. nov., is described from West Malaysia, based on morphological characters. The description includes detailed drawings of the most important structures. Four fragments of DNA, three mitochondrial (cox1, cob, rrnL) and one nuclear (SSU), were sequenced and compared with H. opis Hinton and other elmid species. The taxonomic position of H. belatani sp. nov., its distribution and the phylogeny of the genus are discussed.

10aColeoptera10aDNA10aElmidae10aHedyselmis10aMalaysia10amorphology10anew species10aphylogeny1 aČiampor, F.1 aČiamporová-Zaťovičová, Z. uhttps://elmidae.myspecies.info/node/2602086nas a2200133 4500008004100000245012100041210006900162300001200231490000800243520162600251100001701877700001501894856004301909 2006 eng d00aHedyselmis opis: Description of the larva and its phylogenetic relation to Graphelmis (Coleoptera: Elmidae: Elminae)0 aHedyselmis opis Description of the larva and its phylogenetic re a627-6360 v1033 a

The riffle beetle genus Hedyselmis Hinton, 1976 includes two species from the Malay Peninsula, with adults with a highly deviating morphology. Its phylogenetic relationships are unclear, although it has been hypothesized to be related to Graphelmis Delève, 1968, a large genus widely distributed in the Oriental and East Palaearctic regions. In this paper the larva of H. opis Hinton, 1976 is described based on material collected in the Cameron Highlands (Malaysia) and the conspecificity with co-existing adults tested using sequences of one nuclear (5’ end of 18S rRNA) and three mitochondrial gene fragments (5’ end of the large ribosomal unit + tRNAleu + 5’ end of the NADH dehydrogenase subunit 1; 5’ end of cytochrome c oxidase subunit I; and a fragment of cytochrome b) with a total of ca. 2,600 bp. This is the first example of the use of molecular data to match different life stages within the family Elmidae. The larva of H. opis has a subcylindrical body typical of many other elmid genera; abdominal segments 1–7 with preserved pleura; and ninth segment with oval operculum. The last instar larvae have clearly visible prominent spiracles on mesothorax and abdominal segments 1–8. The phylogenetic position of Hedyselmis in relation to Graphelmis was investigated using molecular data for three species of Graphelmis plus a selection of other Elmidae genera. Hedyselmis opis is nested within Graphelmis, confirming their close relationship and suggesting that their status requires taxonomic revision.

1 aČiampor, F.1 aRibera, I. uhttps://elmidae.myspecies.info/node/24