|Publication Type:||Journal Article|
|Year of Publication:||2020|
|Authors:||H. Freitag, Molls, C., Bouma, A. M., Garces, J. M., Rossato, M., Cosentino, E., Delledonne, M.|
|Journal:||Journal of Natural History|
|Keywords:||Citizen science, nanopore sequencing, riffle beetle, taxon expeditions, taxonomy|
Four new species, Grouvellinus nigerquadratus sp. nov., G. cruxniger sp. nov., G. luciaensis sp. nov., and G. borneensis sp. nov., are described from Borneo and illustrated in detail. Two additional species of the genus which remain undescribed are brieﬂy diagnosed. The material was collected during Taxon Expeditions’ ﬁeld course which involved citizen scientists, students and taxonomists. Specimens were collected at altitudes between 270 m and 750 m above sea level using ﬁne-meshed hand-nets and blacklight traps. The morphological species delimitation based on morphology was supplemented and congruent with mtDNA sequences which are the ﬁrst DNA barcodes for the genus from Borneo. They were obtained in the ﬁeld using a newly developed rapid and accurate MinION-based workﬂow. The inter – and intraspeciﬁc genetic distances and the problems regarding cryptic species delimitation are discussed.